Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Tengis S. Pavlov; Daria V. Ilatovskaya; Oleg Palygin; Vladislav Levchenko; Oleh Pochynyuk; Alexander Staruschenko

doi:10.3791/53035

Journal

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Biology

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Patch Kelepçe ve Canlı Floresan Mikroskobu uygulanması Taze İzole PKD Epitelinin Fonksiyonel Properties Monitör

JoVE Journal
Biology

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JoVE Journal Biology

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Please note that all translations are automatically generated. Click here for the English version.

Patch Kelepçe ve Canlı Floresan Mikroskobu uygulanması Taze İzole PKD Epitelinin Fonksiyonel Properties Monitör

DOI:

10.3791/53035-v

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08:46 min

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September 01, 2015

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Tengis S. Pavlov, Daria V. Ilatovskaya, Oleg Palygin, Vladislav Levchenko, Oleh Pochynyuk, Alexander Staruschenko

¹Department of Physiology,Medical College of Wisconsin, ²Department of Integrative Biology & Pharmacology,University of Texas Health Science Center at Houston

Chapters

00:05Title
02:14Isolation of Renal Cysts and Connecting Tubules/Collecting Duct Segments
05:02Single Channel Patch-clamp Electrophysiology
05:32Ratiometric Epifluorescence Measurement of Intracellular Calcium Concentration in the Epithelial Cells
06:55Results: Effect of Benzamil Treatment and ATP in the Cystic Cells of PCK Rats
08:08Conclusion

Summary

Automatic Translation

Renal tübüler epitel ifade iyon kanalları polikistik böbrek hastalığı patolojisinde önemli bir rol oynamaktadır. Burada taze kemirgen böbrekler izole kistik epitel patch-kelepçe analizi ve hücre içi kalsiyum seviyesi ölçümleri gerçekleştirmek için kullanılan deneysel protokolleri açıklayın.