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Chapters
- 00:05Title
- 01:42Preparation of Double-barreled Ion-selective Microelectrodes
- 04:36Preparation of Concentric Ion-selective Microelectrodes
- 06:56Calibration of Ion-selective and Concentric Ion-selective Microelectrodes
- 07:44Electrophysiology Experiments
- 08:40Results: Calibration of Sodium-selective Microelectrodes and Comparison of Double-barreled Electrode with Concentric Electrode
- 10:31Conclusion
We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.
