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Neuroscience

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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
 

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Article doi: 10.3791/57511
May 7th, 2018

Summary May 7th, 2018

Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

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