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Protocole pour tridimensionnelle confocale Analyse morphométrique des astrocytes
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
DOI:

09:13 min

December 11, 2015

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Chapters

  • 00:05Title
  • 00:46Immunohistochemistry of Rat Brain Slices
  • 02:38Confocal Microscopy
  • 04:51Data Analysis of 2D and 3D Confocal Images
  • 07:17Results: Qualitative and Quantitative Analysis of Astrogliosis
  • 08:19Conclusion

Summary

Automatic Translation

Astrocytes in the CNS change their functional and structural properties in response to harmful stimuli. This report presents a protocol for assessment of three-dimensional astrocyte morphology in diseased conditions or after therapeutic interventions.

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