JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
无标记技术的单细胞分泌物的时空成像
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Please note that all translations are automatically generated.
Click here for the English version.
无标记技术的单细胞分泌物的时空成像
DOI:
10.3791/53120-v
•
09:09 min
•
November 23, 2015
•
Deepa Raghu
,
Joseph A. Christodoulides
,
James B. Delehanty
,
Jeff M. Byers
,
Marc P. Raphael
1
Materials Science and Technology
,
Naval Research Laboratory
,
2
Center for Bio/Molecular Science and Engineering
,
Naval Research Laboratory
Chapters
00:05
Title
01:05
Nanostructure Fabrication
03:23
Chip Cleaning and Application of Self-assembled Monolayer
04:07
LSPR General Settings – Chip Preparation and Mounting
06:39
LSPR Imaging of Anti-c-myc Secretions from 9E10 Hybridoma Cells
07:14
Results: Spatio-temporal Imaging of Single Cell Secretions by LSPRi
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
细胞间的通信为内和细胞外的控制各种生理活性的关键。本文描述了测量的单细胞的分泌物的时空性质的协议。为了实现这一目标,多学科的方法是使用集成了无标记纳米等离子体激感测与活细胞成像。
Tags
Label-free
Single Cell Secretions
Localized Surface Plasmon Resonance Imaging (LSPRi)
Protein Secretion
Cell-to-cell Signaling
Nanofabrication
Biofunctionalization
Binding Kinetics
Live Cell Imaging
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在活细胞中的小GTPase活性时空操纵在亚细胞水平上几秒钟的时间刻度
非接触,无标签使用拉曼光谱研究细胞和细胞外基质的监测
微流体技术,探讨细胞变形
微流体为基础的电化学生物芯片的无标记的DNA杂交分析
无标记单分子检测使用微型环光学谐振器
从混合三维培养使用近红外Photoimmunotherapy技术选择性细胞消除
PIP-on-a-chip:蛋白质 - 磷酸肌醇相互作用的无标记研究
构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南
实现干扰反射显微镜,实现无标签、高速微管成像
使用光纤共聚焦激光微内窥镜检查的眼部药物输送系统的时空
体内
成像
Read Article