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Chapters
- 00:05Title
- 01:54Transfection
- 03:17GST-kinase Pulldown
- 06:57Running, Staining, and Drying Gels and Developing Films
- 09:08Results: Example of a Screen Outcome
- 10:26Conclusion
Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.
