JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
利用共聚焦分析<em>非洲爪蟾</em>调查的Wnt和Shh形态发生素梯度的调节剂
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Using Confocal Analysis of
Xenopus laevis
to Investigate Modulators of Wnt and Shh Morphogen Gradients
Please note that all translations are automatically generated.
Click here for the English version.
利用共聚焦分析<em>非洲爪蟾</em>调查的Wnt和Shh形态发生素梯度的调节剂
DOI:
10.3791/53162-v
•
08:10 min
•
December 14, 2015
•
Simon W. Fellgett
,
Simon A. Ramsbottom
,
Richard J. Maguire
,
Stephen Cross
,
Peter O’Toole
,
Mary E. Pownall
1
Department of Biomedical Science
,
The Bateson Centre, University of Sheffield
,
2
Institute of Genetic Medicine
,
Newcastle University
,
3
Department of Cardiovascular Science
,
The Bateson Centre, University of Sheffield
,
4
School of Biochemistry
,
University of Bristol
,
5
Biology Department
,
University of York
Chapters
00:05
Title
01:09
Microinjecting Xenopus Embryos
02:10
Excising Animal Cap Explants
04:17
Animal Explant Imaging
06:04
Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants
07:38
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该手稿这里提供一组简单的用于分析的在爪蟾荧光标记的配体的分泌和扩散的方法。这提供了一个上下文用于测试的其他蛋白质的修改配体分布的能力,并允许实验,可能会给洞察调节形态发生素梯度的机制。
Tags
Xenopus Laevis
Confocal Analysis
Wnt
Sonic Hedgehog (Shh)
Morphogen Gradients
GFP-tagged Ligands
MRNA Injection
Animal Caps
Lineage Tracing
Fluorescence Imaging
Quantitative Gradient Analysis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em
操作和<em>在体外</em中>成熟<em>非洲爪蟾</em>卵母细胞,其次是胞浆内单精子注射,研究胚胎发育
功能性克隆使用<em>爪</em>卵母细胞表达系统
损失 - 和增益的功能角度进行调查研究早期细胞命运决定在植入前小鼠胚胎
技术目标显微注射到显影<em>爪</em>肾
研究Wnt信号传导过程中的航空公司图案
建立有限元模型,研究斑马鱼下颌生物力学
住发展拟南芥花的共焦成像
胚胎血管生成的可视化和定量分析<em>热带非洲爪蟾</em
将DNA微注射到
异
种胚胎的眼蕾中,以及GFP表达光学轴状轴向轴向的成像,活
的Xenopus
大白鼠
Read Article