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Bruke Confocal Analyse av<em> Xenopus laevis</em> For å undersøke modulatorer av Wnt og Shh Morphogen Gradienter
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1Department of Biomedical Science,The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine,Newcastle University, 3Department of Cardiovascular Science,The Bateson Centre, University of Sheffield, 4School of Biochemistry,University of Bristol, 5Biology Department,University of York
Chapters
- 00:05Title
- 01:09Microinjecting Xenopus Embryos
- 02:10Excising Animal Cap Explants
- 04:17Animal Explant Imaging
- 06:04Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants
- 07:38Conclusion
Manuskriptet her gir et enkelt sett med metoder for å analysere utskillelsen og spredning av fluorescently merkede ligander i Xenopus. Dette gir en sammenheng for å teste muligheten for andre proteiner for å modifisere ligand fordeling og tillater eksperimenter som kan gi innsikt i mekanismene som regulerer morphogen gradienter.
