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利用共聚焦分析<em>非洲爪蟾</em>调查的Wnt和Shh形态发生素梯度的调节剂

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Developmental Biology

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JoVE Journal Developmental Biology

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Please note that all translations are automatically generated. Click here for the English version.

利用共聚焦分析<em>非洲爪蟾</em>调查的Wnt和Shh形态发生素梯度的调节剂

DOI:

10.3791/53162-v

•

08:10 min

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December 14, 2015

•

Simon W. Fellgett, Simon A. Ramsbottom, Richard J. Maguire, Stephen Cross, Peter O’Toole, Mary E. Pownall

¹Department of Biomedical Science,The Bateson Centre, University of Sheffield, ²Institute of Genetic Medicine,Newcastle University, ³Department of Cardiovascular Science,The Bateson Centre, University of Sheffield, ⁴School of Biochemistry,University of Bristol, ⁵Biology Department,University of York

Chapters

00:05Title
01:09Microinjecting Xenopus Embryos
02:10Excising Animal Cap Explants
04:17Animal Explant Imaging
06:04Results: Diffusion Analysis of Fluorescently Tagged Ligands in Xenopus Animal Cap Explants
07:38Conclusion

Summary

Automatic Translation

该手稿这里提供一组简单的用于分析的在爪蟾荧光标记的配体的分泌和扩散的方法。这提供了一个上下文用于测试的其他蛋白质的修改配体分布的能力，并允许实验，可能会给洞察调节形态发生素梯度的机制。

Tags

Xenopus Laevis Confocal Analysis Wnt Sonic Hedgehog (Shh)Morphogen Gradients GFP-tagged Ligands MRNA Injection Animal Caps Lineage Tracing Fluorescence Imaging Quantitative Gradient Analysis

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