Journal
/
/
鸡胚全视网膜外植体电穿孔和化学试剂处理
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
DOI:

09:40 min

September 30, 2015

, ,

Chapters

  • 00:05Title
  • 01:33Egg Handling and Eye Collection
  • 03:21Preparation of Whole Retinal Explants
  • 04:15Electroporation of Whole Retinal Explants
  • 07:28Results: Uses of Cultured Whole Retinal Explants
  • 08:59Conclusion

Summary

Automatic Translation

本协议描述的方法来剖析,实验操作和鸡胚培养全视网膜植。当高的成功率,有效性和再现性都需要测试质粒电穿孔和/或试剂的物质, 即,酶抑制剂的效应的外植体培养是有用的。

Related Videos

Read Article