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鸡胚全视网膜外植体电穿孔和化学试剂处理
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Developmental Biology
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
Please note that all translations are automatically generated.
Click here for the English version.
鸡胚全视网膜外植体电穿孔和化学试剂处理
DOI:
10.3791/53202-v
•
09:40 min
•
September 30, 2015
•
Shahrzad Shirazi Fard
,
Maria Blixt
,
Finn Hallböök
1
Department of Neuroscience, Biomedical Center
,
Uppsala University
Chapters
00:05
Title
01:33
Egg Handling and Eye Collection
03:21
Preparation of Whole Retinal Explants
04:15
Electroporation of Whole Retinal Explants
07:28
Results: Uses of Cultured Whole Retinal Explants
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述的方法来剖析,实验操作和鸡胚培养全视网膜植。当高的成功率,有效性和再现性都需要测试质粒电穿孔和/或试剂的物质,
即,酶
抑制剂的效应的外植体培养是有用的。
Tags
Whole Retinal Explants
Chicken Embryos
Electroporation
Chemical Reagents
Experimental Model
Central Nervous System
Intraocular Injections
Ex Vivo Culture
Controlled Exposure
Rapid
Reproducible
Cost-effective
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