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جيل من المستحثة متعددة القدرات الخلايا الجذعية من الإنسان الطرفية T الخلايا باستخدام سينداي الفيروسات في ظروف خالية من الطاعم
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
DOI:

09:32 min

November 11, 2015

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Chapters

  • 00:05Title
  • 01:19Preparation of Activated Human T Cells
  • 04:05Generating Induced Pluripotent Stem Cells (iPSCs) from Activated T Cells
  • 07:11Expansion of T Cell-derived iPSCs
  • 08:28Results: Generation of iPSCs from Human Peripheral T Cells
  • 09:03Conclusion

Summary

Automatic Translation

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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