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Una rapida e quantitativa metodo fluorimetrico per lo screening di stupefacenti piccole molecole di proteine Targeting
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Chapters
- 00:05Title
- 01:05Synthesis of Protein-templated Gold Nanoclusters (Au NCs)
- 03:17Screen Relative Binding Affinity of Different Small Molecular Drugs to HSA
- 05:19Measure the Binding Constant of a Specific Drug to HSA
- 06:41Results: Studying Protein-drug Binding by Forming Fluorescent Gold Nanoclusters (Au NCs) using Drug-loaded Serum Albumen as Template
- 07:53Conclusion
A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au NCs.
