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人骨髓间充质干细胞（干细胞）的免疫调节性能评估
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
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人骨髓间充质干细胞（干细胞）的免疫调节性能评估

DOI:
10.3791/53265-v

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06:20 min

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December 24, 2015

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, , , ,
1Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), 2National Institute of Cancer Research,National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology,National Defense Medical Center

Chapters

  • 00:05Title
  • 00:44Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 02:23MSC: Leukocyte Co-culture and Leukocyte Activation
  • 03:31Variation: Effector Suppression Assay
  • 04:38Results: Representative Suppressive Effects of MSC on Leukocyte Proliferation
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

人类骨髓间充质干细胞（MSC）的免疫调节属性显示越来越重要的临床应用。用MSC和外周血白细胞的共同培养系统的预染色的荧光染料羧基琥珀酰亚胺酯（CFSE），我们描述对效应白细胞增殖和特定亚群的体外评估的MSC免疫调节的。

Tags

Mesenchymal Stem CellsImmunomodulationLeukocyte ProliferationCFSEFlow CytometryEffector CellsImmune-related Diseases

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