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Immunology and Infection
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Vurdering af immunmodulerende egenskaber af humane mesenchymale stamceller (MSC'er)
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)

Vurdering af immunmodulerende egenskaber af humane mesenchymale stamceller (MSC'er)

DOI:
10.3791/53265-v

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06:20 min

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December 24, 2015

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, , , ,
1Regenerative Medicine Research Group, Institute of Cellular & System Medicine,National Health Research Institutes (NHRI), 2National Institute of Cancer Research,National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology,National Defense Medical Center

Chapters

  • 00:05Title
  • 00:44Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 02:23MSC: Leukocyte Co-culture and Leukocyte Activation
  • 03:31Variation: Effector Suppression Assay
  • 04:38Results: Representative Suppressive Effects of MSC on Leukocyte Proliferation
  • 05:51Conclusion

Summary

Automatic Translation

Automatic Translation

De immunmodulerende egenskaber af humane mesenkymale stamceller (MSC) fremgår mere relevante for klinisk anvendelse. Ved hjælp af en co-dyrkningssystemet ifølge MSC'er og perifere blodleukocytter præ-farvet med det fluorescerende farvestof carboxyfluorescein succinimidyl ester (CFSE), beskriver vi in vitro vurdering af MSC immunmodulation på effektor leukocyt proliferation og specifikke subpopulationer.

Tags

Mesenchymal Stem CellsImmunomodulationLeukocyte ProliferationCFSEFlow CytometryEffector CellsImmune-related Diseases

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