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Descrivendo un regolamento Dipendente Fattore di trascrizione del MicroRNA trascrittoma
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Bioengineering
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JoVE Journal Bioengineering
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Descrivendo un regolamento Dipendente Fattore di trascrizione del MicroRNA trascrittoma

DOI:
10.3791/53300-v

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07:23 min

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June 15, 2016

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, , , , , , ,
1Division of Hematology, Davidoff Cancer Center,Rabin Medical Center, 2The Center of Nanoscience and Nanotechnology,Tel Aviv University, 3Experimental Therapeutics,The University of Texas MD Anderson Cancer Center, 4Department of Leukemia,The University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 01:02Data Mining for Transcription Factor Binding Sites in the Promoter Regions of MicroRNA
  • 02:20Producing a Lentivirus-containing shRNA in 293 Human Embryonic Kidney Cell Line
  • 04:03Transfection of a Cell with Lentivirus-containing shRNA
  • 05:48Results: shRNA Down-regulation of STAT3 MicroRNA Leads to Significant Expression Changes in CLL Cells
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.

Tags

Keywords: Transcription FactorMicroRNA TranscriptomeData MiningBioinformatic AnalysisChromatin Immunoprecipitation SequencingH3K4me3 Epigenetic SignatureMedian Binding AffinityShRNAHEK293 CellsDMEMFBSLentivirusPackaging VectorsTransfection

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