JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
评价肺转移的小鼠乳腺肿瘤模型定量实时荧光定量PCR
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
Please note that all translations are automatically generated.
Click here for the English version.
评价肺转移的小鼠乳腺肿瘤模型定量实时荧光定量PCR
DOI:
10.3791/53329-v
•
08:56 min
•
January 29, 2016
•
Melissa A. Abt
,
Christina L. Grek
,
Gautam S. Ghatnekar
,
Elizabeth S. Yeh
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
FirstString Research, Inc.
Chapters
00:05
Title
00:37
Lung Dissection
02:00
RNA Isolation
05:23
First Strand Synthesis and Real-time PCR
06:57
Results: Quantitative Real-time PCR Evaluation of Mouse Mammary Tumors
08:21
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个协议描述了如何使用定量实时PCR(QRT-PCR),以检测表示内的小鼠肺组织转移的肿瘤细胞特异性mRNA。
Tags
Lung Metastasis
Mouse Mammary Tumor Models
Quantitative Real-time PCR
RNA Isolation
Lysis Buffer
Proteinase K
RNA Precipitation
RNA Column Purification
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用实时活体成像在肿瘤纳米粒子吸收的评价
在小鼠模型
体内
生物发光成像乳腺癌脑转移的肿瘤乏氧动力学
实时定量PCR(qPCR的microRNA检测在前列腺肿瘤)
骨转移模型
实时成像在乳腺癌小鼠模型中的肿瘤微环境对药物的反应
采用实时定量PCR检测供体细胞植入一个具有竞争力的小鼠骨髓移植模型
一个简单的方法小鼠肺插管
乳腺癌的一种新的转基因小鼠模型:转移性乳腺癌通过瞄准导管上皮与腺病毒Cre重组酶的启动
脑脊液的microRNA分析采用实时定量PCR技术
原发灶和MEF细胞分离,研究肺转移
Read Article