Journal
/
/
评价肺转移的小鼠乳腺肿瘤模型定量实时荧光定量PCR
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR
DOI:

08:56 min

January 29, 2016

, , ,

Chapters

  • 00:05Title
  • 00:37Lung Dissection
  • 02:00RNA Isolation
  • 05:23First Strand Synthesis and Real-time PCR
  • 06:57Results: Quantitative Real-time PCR Evaluation of Mouse Mammary Tumors
  • 08:21Conclusion

Summary

Automatic Translation

这个协议描述了如何使用定量实时PCR(QRT-PCR),以检测表示内的小鼠肺组织转移的肿瘤细胞特异性mRNA。

Related Videos

Read Article