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Evaluering af lungemetastase i musebrysttumor modeller af kvantitativ real-time PCR
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JoVE Journal Medicine
Evaluation of Lung Metastasis in Mouse Mammary Tumor Models by Quantitative Real-time PCR

Evaluering af lungemetastase i musebrysttumor modeller af kvantitativ real-time PCR

DOI:
10.3791/53329-v

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08:56 min

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January 29, 2016

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, , ,
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2FirstString Research, Inc.

Chapters

  • 00:05Title
  • 00:37Lung Dissection
  • 02:00RNA Isolation
  • 05:23First Strand Synthesis and Real-time PCR
  • 06:57Results: Quantitative Real-time PCR Evaluation of Mouse Mammary Tumors
  • 08:21Conclusion

Summary

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Denne protokol beskriver, hvordan man bruger kvantitativ real-time PCR (QRT-PCR) til påvisning af tumorcellespecifikke mRNA repræsenterer metastase i mus lungevæv.

Tags

Lung MetastasisMouse Mammary Tumor ModelsQuantitative Real-time PCRRNA IsolationLysis BufferProteinase KRNA PrecipitationRNA Column Purification

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