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Journal
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Neuroscience
/
性别差异在小鼠海马星形胶质细胞<em>在体外</em>缺血
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Sex Differences in Mouse Hippocampal Astrocytes after
In-Vitro
Ischemia
Please note that all translations are automatically generated.
Click here for the English version.
性别差异在小鼠海马星形胶质细胞<em>在体外</em>缺血
DOI:
10.3791/53695-v
•
08:32 min
•
October 25, 2016
•
Vishal Chanana
,
Abdulfettah Tumturk
,
Douglas Kintner
,
Eshwar Udho
,
Peter Ferrazzano
3
,
Pelin Cengiz
3
1
Waisman Center
,
University of Wisconsin
,
2
Department of Neurological Surgery
,
University of Wisconsin
,
3
Department of Pediatrics
,
University of Wisconsin
Chapters
00:05
Title
00:42
Hippocampal Dissection and Plating
03:32
Astrocyte Culture
05:34
Oxygen and Glucose Deprivation and Reoxygenation (OGD/REOX)
06:21
Results: Increased Protein Expression of HIF1α and Astrocytic Markers Following OGD/REOX
07:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
星形胶质细胞是中枢神经系统(CNS)最重要的关键球员之一。在这里,我们报告,以研究男性和女性新生儿幼崽的星形胶质细胞功能
的体外
缺血后背后的机制性控海马星形胶质细胞培养协议的实用方法。
Tags
Mouse
Hippocampal
Astrocytes
In-vitro
Ischemia
Sex Differences
Cell Culture
Oxygen And Glucose Deprivation
Reoxygenation
Hypoxic Ischemia
Newborn
Brain Dissection
Trypsin
Astrocyte Plating Medium
Cell Suspension
Article
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