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JoVE Journal Developmental Biology
Feeder-fria Derivering av Melanocyter från Human pluripotenta stamceller
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Feeder-fria Derivering av Melanocyter från Human pluripotenta stamceller

Article DOI: 10.3791/53806 • 12:21 min • March 3rd, 2016
March 3rd, 2016 •
1,2, 3, 1
1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

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Detta arbete beskriver ett in vitro differentiering protokoll för att producera pigmente, mogna melanocyter från humana pluripotenta stamceller via en neural crest och melanoblast mellansteg med hjälp av en matarfria, 25 dagars protokoll.

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Utvecklingsbiologi melanocyter pigmentering melanoblast embryonala stamceller (ESC) humana pluripotenta stamceller (hPSCs) inducerade pluripotenta stamceller (iPSCs) Neural Crest in vitro differentiering sjukdom modellering differentiering protokoll mänskliga embryonala stamceller
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