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高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代
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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
Please note that all translations are automatically generated.
Click here for the English version.
高通量CRISPR载体的构建及DNA修饰的表征番茄毛状根的一代
DOI:
10.3791/53843-v
•
12:59 min
•
April 30, 2016
•
Thomas B. Jacobs
,
Gregory B. Martin
2
1
Boyce Thompson Institute for Plant Research
,
2
Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science
,
Cornell University
Chapters
00:05
Title
00:36
CRISPR Vector Construction
06:36
Hairy Root Transformation
10:59
Results: Hairy Root Cultures and Detection of DNA Modification
12:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用DNA装配,可以并行地构建在单个克隆反应多个CRISPR矢量,使得大量CRISPR的建设矢量a简单的任务。番茄毛状根是一个很好的模型系统来验证CRISPR载体和产生突变体材料。
Tags
CRISPR
Vector Construction
DNA Modifications
Tomato Hairy Roots
Functional Genomics
Cloning Procedure
Guide RNA
Knockout Mutants
PCR Amplification
High-Throughput
DNA Assembly
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