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Chapters
- 00:05Title
- 01:11Isolation of Germinal Vesicles
- 02:53Removal of Frog Nuclear Envelopes
- 04:25Removal of Salamander Nuclear Envelopes
- 05:17Observation of Lampbrush Chromosomes and Centrifugation of Nuclear Contents
- 06:33Fixing the Nuclear Contents
- 07:30Results: Visualization of Axolotl and Frog LBCs
- 09:14Conclusion
Vi presenterer enkle teknikker for å isolere gigantiske transcriptionally aktive lampbrush kromosomer fra å leve oocytter av frosker og salamandere. Vi beskriver hvordan observere disse kromosomene "live" av fase kontrast eller differensial interferens kontrast, og hvordan du løser dem for fluorescerende in situ hybridisering eller immunofluorescent farging.
