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Isolering av Giant Lampbrush Kromosomer fra Living oocytter av frosker og salamandere
JoVE Journal
Genetics
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JoVE Journal Genetics
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
DOI:

10:07 min

December 05, 2016

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Chapters

  • 00:05Title
  • 01:11Isolation of Germinal Vesicles
  • 02:53Removal of Frog Nuclear Envelopes
  • 04:25Removal of Salamander Nuclear Envelopes
  • 05:17Observation of Lampbrush Chromosomes and Centrifugation of Nuclear Contents
  • 06:33Fixing the Nuclear Contents
  • 07:30Results: Visualization of Axolotl and Frog LBCs
  • 09:14Conclusion

Summary

Automatic Translation

Vi presenterer enkle teknikker for å isolere gigantiske transcriptionally aktive lampbrush kromosomer fra å leve oocytter av frosker og salamandere. Vi beskriver hvordan observere disse kromosomene "live" av fase kontrast eller differensial interferens kontrast, og hvordan du løser dem for fluorescerende in situ hybridisering eller immunofluorescent farging.

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