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从青蛙和蝾螈的卵母细胞活巨Lampbrush染色体的分离
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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Please note that all translations are automatically generated.
Click here for the English version.
从青蛙和蝾螈的卵母细胞活巨Lampbrush染色体的分离
DOI:
10.3791/54103-v
•
10:07 min
•
December 05, 2016
•
Joseph G. Gall
,
Zehra F. Nizami
1
Department of Embryology
,
Carnegie Institution for Science
Chapters
00:05
Title
01:11
Isolation of Germinal Vesicles
02:53
Removal of Frog Nuclear Envelopes
04:25
Removal of Salamander Nuclear Envelopes
05:17
Observation of Lampbrush Chromosomes and Centrifugation of Nuclear Contents
06:33
Fixing the Nuclear Contents
07:30
Results: Visualization of Axolotl and Frog LBCs
09:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出简单的技术从活青蛙和蝾螈卵母细胞中分离巨头转录活性lampbrush染色体。我们描述了如何通过相位对比或微分干涉对比,以及如何解决这些问题
原位
杂交和免疫荧光染色荧光观察这些染色体“活着”。
Tags
Giant Lampbrush Chromosomes
Amphibian Oocyte
Germinal Vesicle
Nuclear Organelles
Transcription
Splicing
RNA Export
Immunofluorescence
In Situ Hybridization
Frog
Salamander
Oocyte Culture Medium
GV Isolation Medium
Dispersal Medium
Nuclear Envelope
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