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从青蛙和蝾螈的卵母细胞活巨Lampbrush染色体的分离
JoVE Journal
Genetics
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JoVE Journal Genetics
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
DOI:

10:07 min

December 05, 2016

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Chapters

  • 00:05Title
  • 01:11Isolation of Germinal Vesicles
  • 02:53Removal of Frog Nuclear Envelopes
  • 04:25Removal of Salamander Nuclear Envelopes
  • 05:17Observation of Lampbrush Chromosomes and Centrifugation of Nuclear Contents
  • 06:33Fixing the Nuclear Contents
  • 07:30Results: Visualization of Axolotl and Frog LBCs
  • 09:14Conclusion

Summary

Automatic Translation

我们提出简单的技术从活青蛙和蝾螈卵母细胞中分离巨头转录活性lampbrush染色体。我们描述了如何通过相位对比或微分干涉对比,以及如何解决这些问题原位杂交和免疫荧光染色荧光观察这些染色体“活着”。

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