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A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
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A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

DOI:
10.3791/54136-v

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09:37 min

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July 14, 2016

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, , , , ,
1Department of Environmental Health Sciences,Yale University School of Medicine, 2Department of Ophthalmology,Yale University School of Medicine

Chapters

  • 00:05Title
  • 00:47Neonatal Hind Limb Test
  • 02:31Weanling Observation Test
  • 04:26Wire Mesh Grip Strength Test
  • 06:15Tissue Staining: NADH Diaphorase or Succinate Dehydrogenase
  • 07:47Results: HTRA2-/- Mice Testing and Histology
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

A reverse-genetics approach to understanding gene families associated with human disease is presented, using mouse as a model system, and the subsequent mouse phenotyping schedule is described. Because mice defective in a gene of interest, HtrA2, manifested Parkinsonian symptoms, the phenotyping regimen is focused on identifying neurological defects.

Tags

Keywords: PhenotypingGenetically Modified MiceAgingHind Limb TestWeanling Observation TestProximal Hind Limb Muscle StrengthMuscle WeaknessMuscle FatigueBehavioral PhenotypesCellular PhenotypesHuman Disease ModelsMouse ModelsAcclimationWeight RecordingVideo RecordingStandardized Testing Setup

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