A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Victoria L. Patterson; Brian S. Thompson; Catherine Cherry; Shao-bin Wang; Bo Chen; Josephine Hoh

doi:10.3791/54136

Journal

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Medicine

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وPhenotyping حمية لالفئران المعدلة وراثيا المستخدمة لدراسة الجينات المتورطة في الأمراض البشرية الشيخوخة

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

JoVE Journal
Medicine

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JoVE Journal Medicine

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

وPhenotyping حمية لالفئران المعدلة وراثيا المستخدمة لدراسة الجينات المتورطة في الأمراض البشرية الشيخوخة

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8,189 Views

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09:37 min

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July 14, 2016

DOI:

10.3791/54136-v

DOI:

10.3791/54136-v

•

09:37 min

July 14, 2016

•

3 Views

Victoria L. Patterson*¹, Brian S. Thompson*¹, Catherine Cherry*¹, Shao-bin Wang², Bo Chen², Josephine Hoh^1,2

¹Department of Environmental Health Sciences,Yale University School of Medicine, ²Department of Ophthalmology,Yale University School of Medicine

Transcript

Summary

A reverse-genetics approach to understanding gene families associated with human disease is presented, using mouse as a model system, and the subsequent mouse phenotyping schedule is described. Because mice defective in a gene of interest, HtrA2, manifested Parkinsonian symptoms, the phenotyping regimen is focused on identifying neurological defects.