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生长在微重力人体肠道黏膜的多细胞三维器官模型的建立
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Bioengineering
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JoVE Journal Bioengineering
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity
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生长在微重力人体肠道黏膜的多细胞三维器官模型的建立

DOI:
10.3791/54148-v

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08:54 min

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July 25, 2016

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, ,
1Center for Vaccine Development, Department of Pediatrics,University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital for Children

Chapters

  • 00:05Title
  • 00:55Preparation of Culture Vessels
  • 01:38Preparation of Cell Suspension
  • 02:49Preparation of Cell-containing Collagen Gels
  • 07:33Results: 3D Organotypic Model of Human Instestinal Mucosa
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

细胞在三维（3-D）的环境中生长代表的2-D的环境中（ 例如 ，瓶或菜）在细胞培养的显着改进。这里，我们描述根据通过旋转壁上容器（RWV）生物反应器提供微重力培养人肠粘膜的多细胞三维器官模型的发展。

Tags

3D Organotypic ModelHuman Intestinal MucosaMicrogravityRotating Wall Vessel BioreactorsEndothelial CellsFibroblastsCollagenLamininFibronectinHeparin Sulfate ProteoglycanCell Culture

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