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Udvikling af en flercellet Tre-dimensionel organotypisk Model af menneskelige tarmslimhinden Grown Under Microgravity
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Bioengineering
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JoVE Journal Bioengineering
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity

Udvikling af en flercellet Tre-dimensionel organotypisk Model af menneskelige tarmslimhinden Grown Under Microgravity

DOI:
10.3791/54148-v

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08:54 min

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July 25, 2016

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, ,
1Center for Vaccine Development, Department of Pediatrics,University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital for Children

Chapters

  • 00:05Title
  • 00:55Preparation of Culture Vessels
  • 01:38Preparation of Cell Suspension
  • 02:49Preparation of Cell-containing Collagen Gels
  • 07:33Results: 3D Organotypic Model of Human Instestinal Mucosa
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Celler, der vokser i en tredimensional (3-D) miljø repræsenterer en markant forbedring i forhold til celledyrkning i 2-D miljøer (f.eks kolber eller retter). Her beskriver vi udviklingen af ​​en flercellet 3-D organotypiske model af den humane intestinale mucosa dyrket under vægtløshed billede ved at dreje-væg-beholder (RWV) bioreaktorer.

Tags

3D Organotypic ModelHuman Intestinal MucosaMicrogravityRotating Wall Vessel BioreactorsEndothelial CellsFibroblastsCollagenLamininFibronectinHeparin Sulfate ProteoglycanCell Culture

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