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Journal
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Immunology and Infection
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一种简单有效的方法构建突变牛痘病毒载体
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
Please note that all translations are automatically generated.
Click here for the English version.
一种简单有效的方法构建突变牛痘病毒载体
DOI:
10.3791/54171-v
•
09:16 min
•
October 30, 2016
•
Ming Yuan*
1
,
Pengju Wang*
2,3
,
Louisa S. Chard
,
Nicholas R. Lemoine
2,3
,
Yaohe Wang
2,3
1
Center for Molecular Oncology, Barts Cancer Institute
,
Queen Mary University of London
,
2
Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy
,
Zhengzhou University
,
3
School of Basic Medical Sciences, Academy of Medical Sciences
,
Zhengzhou University
Chapters
00:05
Title
00:49
Preparing the CV-1 Cells
02:32
Purification of the Modified Vaccinia Virus (VV)
05:23
Expanding a Purified Plaque for PCR Testing
06:52
Results: VV N1L Modification
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
牛痘病毒(VV)已被广泛用于生物医学研究和人体健康的改善。本文介绍使用CRISPR-Cas9系统编辑VV基因组的简单,高效的方法。
Tags
CRISPR-Cas9
Mutant Vaccinia Virus
Viral Vector
Gene Therapy
Vaccination
CV-1 Cells
Transfection
Shuttle Donor Vector
Plaque Purification
RFP-positive Plaques
Cell Lysis
Virus Dilution
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