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Immunology and Infection
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应用荧光共振能量转移(FRET)审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by
Coxiella burnetii
during siRNA Silencing
Please note that all translations are automatically generated.
Click here for the English version.
应用荧光共振能量转移(FRET)审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中
DOI:
10.3791/54210-v
•
10:29 min
•
July 06, 2016
•
Patrice Newton
,
Eleanor A. Latomanski
,
Hayley J. Newton
1
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity
,
University of Melbourne
Chapters
00:05
Title
01:02
Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
04:25
Infection of Reverse Transfected Cells
05:39
Addition of BlaM Substrate to Determine Level of Translocation
08:23
Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
09:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
调查细菌病原体和宿主之间的相互作用是生物学研究的一个重要领域。在这里,我们描述了必要的技术测量使用布拉姆基板的siRNA的基因沉默过程中
贝氏柯克斯体
效应易位。
Tags
FRET
Effector Translocation
SiRNA Silencing
Coxiella Burnetii
Cellular Microbiology
Host-pathogen Interactions
Chlamydia
Salmonella
E. Coli
Beta-lactamase
ACCM2
HeLa Cells
Transfection
96-well Plate
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