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应用荧光共振能量转移（FRET）审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中

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Immunology and Infection

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JoVE Journal Immunology and Infection

Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

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应用荧光共振能量转移（FRET）审查效应转运效率由<em>贝氏柯克斯体</em> siRNA沉默中

DOI:

10.3791/54210-v

•

10:29 min

•

July 06, 2016

•

Patrice Newton, Eleanor A. Latomanski, Hayley J. Newton

¹Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity,University of Melbourne

Chapters

00:05Title
01:02Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
04:25Infection of Reverse Transfected Cells
05:39Addition of BlaM Substrate to Determine Level of Translocation
08:23Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
09:37Conclusion

Summary

Automatic Translation

调查细菌病原体和宿主之间的相互作用是生物学研究的一个重要领域。在这里，我们描述了必要的技术测量使用布拉姆基板的siRNA的基因沉默过程中贝氏柯克斯体效应易位。

Tags

Keywords: FRET Effector Translocation SiRNA Silencing Coxiella Burnetii Cellular Microbiology Host-pathogen Interactions Chlamydia Salmonella E. Coli Beta-lactamase ACCM2 HeLa Cells Transfection 96-well Plate

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