Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Konark Mukherjee; Helen R. Clark; Vrushali Chavan; Emily K. Benson; Grahame J. Kidd; Sarika Srivastava

doi:10.3791/54214

Journal

/

Neuroscience

/

Analisi di Brain mitocondri Utilizzando Serial Block-Face Scanning Electron Microscopy

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Analisi di Brain mitocondri Utilizzando Serial Block-Face Scanning Electron Microscopy

Please note that all translations are automatically generated. Click here for the English version.

13,990 Views

•

07:47 min

•

July 09, 2016

DOI:

10.3791/54214-v

DOI:

10.3791/54214-v

•

07:47 min

July 09, 2016

•

1 Views

Konark Mukherjee¹, Helen R. Clark¹, Vrushali Chavan¹, Emily K. Benson², Grahame J. Kidd², Sarika Srivastava¹

¹Virginia Tech Carilion Research Institute, ²Renovo Neural Incorporated

Transcript

Summary

Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.