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1Molecular Imaging and Neurovascular Research Laboratory,Dongguk University College of Medicine, 2Biomedical Research Center,Korea Institute of Science and Technology, 3Research Institute of Advanced Materials, Department of Materials Science and Engineering,Seoul National University, 4Departments of Radiology and Cancer Systems Imaging,University of Texas M.D. Anderson Cancer Center
Chapters
- 00:05Title
- 01:20Exogenously Formed Clot Preparation and Fluorescent Marker Labeling
- 02:55Thromboembolic Stroke Model Preparation
- 05:42In Vivo MicroCT Cerebral Thrombus Imaging
- 06:36Ex Vivo Near Infrared Fluorescent (NIRF) Thrombus Imaging and MicroCT Thrombus Area Imaging Quantification
- 09:48Results: Representative Direct Cerebral Thrombus Imaging before and after Thrombolysis Activation
- 11:28Conclusion
Dit protocol beschrijft de toepassing van gecombineerde nabij-infrarood fluorescerende (NIRF) beeldvorming en micro-computertomografie (microCT) voor het visualiseren van cerebrale thromboemboli. Deze techniek maakt de kwantificering van trombus last en evolutie. De NIRF beeldvormende techniek visualiseert fluorescent gelabelde trombus in weggesneden hersenen, terwijl de microCT techniek visualiseert trombus in levende dieren met behulp van goud-nanodeeltjes.
