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Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
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Bioengineering
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JoVE Journal Bioengineering
Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

DOI:
10.3791/54331-v

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10:18 min

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August 27, 2016

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, , , , , , ,
1School of Materials Science and Engineering,Nanyang Technological University, 2School of Chemical and Biomedical Engineering,Nanyang Technological University

Chapters

  • 00:05Title
  • 00:58Fabrication of Polystyrene Microbead Molds
  • 02:48Formation of Bare and ECM-coated PEGDA Scaffolds
  • 06:39Huh-7.5 Cell Culture and Seeding
  • 08:17Results: An Effective 3D Cell Culture Platform for Liver Tissue Engineering
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.

Tags

Keywords: Inverted Colloidal CrystalPoly(ethylene Glycol)3D Cell CultureExtracellular MatrixDrug TestingStem CellsPolystyrene MicrospheresPEGDAPhoto-initiator

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