Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Article DOI: 10.3791/54331-v 10:18 min August 27th, 2016
August 27th, 2016



This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.


Inverted Colloidal Crystal Scaffold PEG-based Scaffold Liver Tissue Engineering 3D Cell Culture Platform Extracellular Matrix Drug Testing Stem Cells Micro-bead Molds Polystyrene Microspheres Ethanol Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter