Journal
/
/
Jämförelse av tobak värdcell Protein borttagning metoder genom Blanche Intakta växter eller genom värmebehandling av extrakt
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
DOI:

11:06 min

August 08, 2016

, ,

Chapters

  • 00:05Title
  • 00:43Heat Precipitation of Tobacco Leaves by Blanching
  • 02:58Heat Precipitation in a Stirred Vessel
  • 04:53Heat Precipitation in a Heat Exchanger and Bag Filtration of Plant Extract
  • 07:55Results
  • 10:09Conclusion

Summary

Automatic Translation

Three heat precipitation methods are presented that effectively remove more than 90% of host cell proteins (HCPs) from tobacco extracts prior to any other purification step. The plant HCPs irreversibly aggregate at temperatures above 60 °C.

Related Videos

Read Article