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通过热烫完整植株或提取物热处理烟草宿主细胞蛋白去除方法的比较
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JoVE Journal Biology
Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts
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通过热烫完整植株或提取物热处理烟草宿主细胞蛋白去除方法的比较

DOI:
10.3791/54343-v

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11:06 min

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August 08, 2016

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, ,
1Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V., 2Institute for Molecular Biotechnology,RWTH Aachen University, 3Department of Biomolecular Separation Engineering,Karlsruhe Institute of Technology (KIT)

Chapters

  • 00:05Title
  • 00:43Heat Precipitation of Tobacco Leaves by Blanching
  • 02:58Heat Precipitation in a Stirred Vessel
  • 04:53Heat Precipitation in a Heat Exchanger and Bag Filtration of Plant Extract
  • 07:55Results
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

之前的任何其他纯化步骤三个换热沉淀方法都能够有效地除去90％以上的烟草宿主细胞蛋白质（医疗专业人员）的提取物。植物医疗专业人员不可逆聚合在温度高于60℃。

Tags

TobaccoHost Cell ProteinsHeat TreatmentBlanchingProtein RemovalPurificationRecombinant BiopharmaceuticalsProteasesExtractionWater BathTemperature ControlIncubation Time

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