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Chapters
- 00:05Title
- 01:10Brain and Spinal Cord Immune Cell Isolation
- 02:57Central Nervous System (CNS) CD4+ T Cell Phenotype Assessment
- 04:44Quantification of Reactive Gliosis Using Mean Fraction Area and Myelin Using Optical Density
- 06:52Results: System Xc-transporter Inhibition Attenuates T Cell CNS Infiltration and Myelin Destruction
- 08:31Conclusion
This protocol describes how to determine whether pharmacological treatments for experimental autoimmune encephalomyelitis show CNS protection as a consequence of suppressing immune cell infiltration or are neuroprotective during the onslaught of immune cell infiltration.
