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1Centre for Translational Omics, Genetics and Genomic Medicine Deptartment, Great Ormond Street Institute of Child Health,University College London, 2Dementia Research Centre, Institute of Neurology,University College London, 3Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy,University of Gothenburg, 4Neurology Unit, Department of Pathophysiology and Transplantation,University of Milan, 5Great Ormond Street Hospital for Children,University College London
Chapters
- 00:05Title
- 01:05Preparation of Standard Peptides
- 03:33LC-MRM Method Development and Data Analysis
- 05:12Results: High-throughput, Multiplex CSF Assay for Quantification of Neurodegeneration Markers and Detection of ApoE Isoforms
- 06:17Conclusion
Wir beschreiben ein Hochdurchsatz-Multiplex, und gezielte proteomic Cerebrospinalflüssigkeit (CSF) -Assay mit Potential für klinische Umsetzung entwickelt. Der Test kann für Neurodegeneration, wie Apolipoprotein E-Varianten (E2, E3 und E4), und messen deren allelische Expression potentielle Marker und Risikofaktoren zu quantifizieren.
