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通过通过纳米孔可调纳米易位流速Zeta电位测定:利用DNA修饰颗粒为例
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Bioengineering
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JoVE Journal
Bioengineering
Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
Please note that all translations are automatically generated.
Click here for the English version.
通过通过纳米孔可调纳米易位流速Zeta电位测定:利用DNA修饰颗粒为例
DOI:
10.3791/54577-v
•
08:42 min
•
October 26, 2016
•
Emma L. C. J. Blundell
,
Robert Vogel
3
,
Mark Platt
1
Department of Chemistry, School of Science
,
Loughborough University
,
2
Izon Science Limited
,
3
School of Mathematics and Physics
,
The University of Queensland
Chapters
00:05
Title
01:07
Preparing Streptavidin Coated Particles and Addition of Capture Probe DNA
02:45
Hybridizing Complementary DNA to the Capture Probe-particles
03:46
TRPS Setup
04:58
Calibrating the Nanopore for Zeta Analysis and Running a Sample
06:43
Results: Size and Zeta Potential of Streptavidin Coated Nanoparticles by TRPS
07:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们使用集成到一个电阻脉冲感测技术经由粒子易位速度的测量,这可以被用来确定各个纳米粒子的ζ电位来表征纳米颗粒表面化学的聚氨酯可调谐纳米孔。
Tags
Zeta Potential
Nanoparticle
Tunable Nanopore
DNA-modified Particles
TRPS
Particle-by-particle Analysis
Bioassay
Streptavidin-coated Particles
DNA Binding
Magnetic Separation
Target DNA Binding
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