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Bestemmelse af Zeta Potentielle via Nanopartikel Translokation Hastigheder gennem en Tunable nanopore: Brug af DNA-modificerede Partikler som eksempel
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
DOI:

08:42 min

October 26, 2016

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Chapters

  • 00:05Title
  • 01:07Preparing Streptavidin Coated Particles and Addition of Capture Probe DNA
  • 02:45Hybridizing Complementary DNA to the Capture Probe-particles
  • 03:46TRPS Setup
  • 04:58Calibrating the Nanopore for Zeta Analysis and Running a Sample
  • 06:43Results: Size and Zeta Potential of Streptavidin Coated Nanoparticles by TRPS
  • 07:47Conclusion

Summary

Automatic Translation

Her bruger vi en polyurethan afstemmelig nanopore integreret i en resistiv puls sensing teknik til at karakterisere nanopartikler overfladekemi via målingen af ​​partikel- translokation hastigheder, som kan anvendes til at bestemme zetapotentialet af individuelle nanopartikler.

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