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細菌の付着と血清抵抗性ビトロネクチンの役割を研究するための試金
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
DOI:

10:55 min

October 16, 2018

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Chapters

  • 00:04Title
  • 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
  • 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
  • 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
  • 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
  • 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
  • 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
  • 08:29Results: Interaction of Vitronectin and Bacteria
  • 10:10Conclusion

Summary

Automatic Translation

このレポートでは、細菌の外膜タンパク質とひと補体レギュレータ ビトロネクチンの相互作用を特徴付けるためのプロトコルについて説明します。この結合反応およびビトロネクチン値の任意の細菌種の生物学的機能を研究するプロトコルを使用できます。

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