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Journal
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Immunology and Infection
/
Vitronectin 在细菌黏附和血清耐药性中作用的实验研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Please note that all translations are automatically generated.
Click here for the English version.
Vitronectin 在细菌黏附和血清耐药性中作用的实验研究
DOI:
10.3791/54653-v
•
10:55 min
•
October 16, 2018
•
Birendra Singh
2
,
Maryam Mostajeran
,
Yu-Ching Su
,
Tamim Al-Jubair
,
Kristian Riesbeck
1
Clinical Microbiology, Department of Translational Medicine
,
Lund University
,
2
Department of Molecular Biology
,
Umea University
Chapters
00:04
Title
00:49
Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
02:18
ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
04:11
Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
04:33
Bacterial Adherence to the Vn-coated Glass Surfaces
05:32
Vn-dependent Adherence of Bacteria to Epithelial Cells
07:29
Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
08:29
Results: Interaction of Vitronectin and Bacteria
10:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本报告描述了用于表征细菌外膜蛋白与人体补体调节器 vitronectin 之间相互作用的协议。该协议可用于研究 vitronectin 在任何细菌物种中的结合反应和生物学功能。
Tags
Vitronectin
Bacterial Adhesion
Serum Resistance
Host-pathogen Interaction
Complement Inhibitor
Flow Cytometry
ELISA
Antihuman Vitronectin Antibody
FITC-conjugated Antibody
Recombinant PH Protein
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