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Journal
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Immunology and Infection
/
方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Please note that all translations are automatically generated.
Click here for the English version.
方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成
DOI:
10.3791/54667-v
•
10:58 min
•
March 29, 2017
•
Graham Brogden*
1,2
,
Ariane Neumann*
1,3
,
Diab M. Husein
,
Friederike Reuner
4
,
Hassan Y. Naim
,
Maren von Köckritz-Blickwede
4
1
Department of Physiological Chemistry
,
University of Veterinary Medicine Hannover
,
2
Fish Disease Research Unit
,
University of Veterinary Medicine
,
3
Department of Clinical Sciences, Biomedical Center
,
Lund University
,
4
Research Center for Emerging Infections and Zoonoses (RIZ)
,
University of Veterinary Medicine Hannover
Chapters
00:05
Title
01:14
Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
03:59
Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
05:33
High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
07:53
High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
08:48
Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
09:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
脂质是已知在细胞功能中起重要作用。在这里,我们描述了一种方法,以确定嗜中性粒细胞的脂质组合物,重点对胆固醇水平,同时使用HPTLC和HPLC纯化,获得更好的理解嗜中性粒细胞的胞外圈闭形成的基本机制的。
Tags
Lipid Alteration
Neutrophil
Neutrophil Extracellular Trap
Thin Layer Chromatography
HPLC
Cell Isolation
Blood-derived Neutrophils
Mononuclear Cells
Polymorphonuclear Cells
Erythrocyte Lysis
Cell Counting
Cell Suspension
Pharmacological Treatment
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