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Journal / Immunology and Infection / Métodos de estudo de lipídios Alterações nos…
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Immunology and Infection

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Métodos de estudo de lipídios Alterações nos neutrófilos ea subsequente formação de neutrófilos Extracelular Traps

Article DOI: 10.3791/54667-v • 10:58 min • March 29th, 2017
March 29th, 2017 •
*1,2, *1,3, 1, 1,4, 1, 1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
* These authors contributed equally

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Lipídios são conhecidos por desempenhar um papel importante nas funções celulares. Aqui, descrevemos um método para determinar a composição lipídica de neutrófilos, com ênfase sobre o nível de colesterol, usando tanto HPTLC e HPLC para obter uma melhor compreensão dos mecanismos subjacentes da formação armadilha extracelular de neutrófilos.

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Immunology edição 121 neutrófilos Neutrófilos Extracelular Armadilhas (NET) colesterol Metil-β-ciclodextrina (MβCD) Cromatografia Líquida de Alta Eficiência (HPLC) de Alto Desempenho A cromatografia em camada fina (HPTLC)
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