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分析蛋白导入进强调,从植物中分离叶绿体
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Please note that all translations are automatically generated.
Click here for the English version.
分析蛋白导入进强调,从植物中分离叶绿体
DOI:
10.3791/54717-v
•
10:18 min
•
November 01, 2016
•
Qihua Ling
,
Paul Jarvis
1
Department of Plant Sciences
,
University of Oxford
Chapters
00:05
Title
00:46
Growth of Arabidopsis Plants and the Stress Treatment
01:34
Chloroplast Isolation
05:06
Analysis of the Yield and the Intactness of Chloroplasts
06:19
Chloroplast Protein Import
08:04
Results: Chloroplast Import of the PsaD Protein Is Elevated in the
sp1
Mutant under Stress Conditions
09:12
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个新的方法来研究蛋白导入叶绿体中分离的压力下。该方法是快速和简单的,并且可以应用于研究的叶绿体蛋白质导入不同胁迫条件的后果,以及相应的调节机制。
Tags
Protein Import
Chloroplasts
Stressed Plants
Arabidopsis Thaliana
Protein Import Regulation
Chloroplast Isolation
Percoll Gradient
Protein Import Assay
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