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Bestemmelse af den relative celleoverfladen og Total ekspression af rekombinante Ion Channels anvendelse af flowcytometri
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JoVE Journal Biology
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Bestemmelse af den relative celleoverfladen og Total ekspression af rekombinante Ion Channels anvendelse af flowcytometri

DOI:
10.3791/54732-v

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11:32 min

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September 28, 2016

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, , , ,
1Département de Physiologie Moléculaire et Intégrative,Montreal Heart Institute Research Centre, 2Département de Microbiologie, Infectiologie, Immunologie,Centre de recherche de l’Hôpital Maisonneuve-Rosemont

Chapters

  • 00:05Title
  • 01:08Liposome Mediated Transient Transfection
  • 02:35Preparing Cell Samples for Staining
  • 04:51Extracellular Staining of Cell Samples
  • 06:10Intracellular Staining of Cell Samples
  • 07:51Data Analysis
  • 08:55Results: Simultaneaous Mutations of N-glycosylation Sites Disrupted Cell Surface Expression of CaVα2δ1
  • 10:25Conclusion

Summary

Automatic Translation

Automatic Translation

Nedarvede hjertearytmier er ofte forårsaget af mutationer, som ændrer overfladen tilførsel af ét eller flere ionkanaler. Her, tilpasser vi flowcytometri assays til tilvejebringelse af en kvantificering af den relative totale og celleoverfladeprotein ekspression af rekombinante ionkanaler udtrykkes i Tsa-201 celler.

Tags

Flow CytometryRecombinant Ion ChannelsCell Surface ExpressionMembrane ProteinsFluorescent AssayCardiac ArrhythmiasMCherryHemagglutinin EpitopeTSA201 CellsTransfectionTrypsin-EDTA

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