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Immunology and Infection
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A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

DOI:
10.3791/54771-v

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09:45 min

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January 25, 2017

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, , , , ,
1Interactions Arbres – Microorganismes, UMR1136,INRA Université de Lorraine, 2Ecologie et Ecophysiologie Forestières – PTEF, UMR 1137,INRA Université de Lorraine, 3Biosciences Division,Oak Ridge National Laboratory

Chapters

  • 00:05Title
  • 00:50Preparation of Bacterial Fungal Cultures
  • 02:11Preparation of In Vitro Biofilm of Bacteria on the Fungal Colony
  • 03:41Sample Preparation for Confocal Microscopy Analysis of Biofilm Formation
  • 05:35Confocal and Electron Microscopic Analysis
  • 07:07Results: Meso- and Micro-scale Analysis of P. fluorescens BBc6 on L. bicolor
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a new method to grow and qualitatively analyze bacterial biofilms on fungal hyphae by confocal and electron microscopy.

Tags

Keywords: Bacterial BiofilmFilamentous Fungal ColoniesConfocal MicroscopyElectron MicroscopyMulti-scale AnalysisCellophane MembraneEDTAFungal Pre-cultureP. FluorescensPotassium Phosphate Buffer

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