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在线体积排阻和离子交换色谱法SAXS光束线
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Please note that all translations are automatically generated.
Click here for the English version.
在线体积排阻和离子交换色谱法SAXS光束线
DOI:
10.3791/54861-v
•
11:09 min
•
January 05, 2017
•
Martha E. Brennich
,
Adam R. Round
3
,
Stephanie Hutin
1
Structural Biology Group
,
European Synchrotron Radiation Facility
,
2
European Molecular Biology Laboratory
,
3
School of Chemical and Physical Sciences
,
Keele University
,
4
Groupe de Microscopie Electronique et Méthodes
,
Institut de Biologie Structurale
Chapters
00:05
Title
00:51
Size-exchange Chromatography (SEC)-Small Angle X-ray Scattering (SAXS) Data Collection
03:46
Ion-exchange Chromatography (IEC)-SAXS Data Collection
07:37
SAXS Data Reduction and Analysis
08:59
Results: SEC- and IEC-SAXS Analysis of D5
323-785
09:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
通过小角X射线散射(SAXS)的蛋白的溶液结构的确定需要单分散样品。在这里,我们提出了两种可能性,以确保样品制备和数据采集之间的最小延迟:在线尺寸排阻色谱(SEC)和在线离子交换层析(IEC)。
Tags
Online Size-exclusion Chromatography
Ion-exchange Chromatography
SAXS Beamline
Structural Biology
SAXS
Molar Dispersed Samples
SEC
Gel Filtration Buffer
HPLC System
SAXS Flow-through Capillary
Radiation Damage
Protein Sample
Aggregates
HPLC Compatible Glass Vial
UV Vis Data
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