Journal
/
/
用GFP标记TMEM184A构建肝素受体身份确认
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
DOI:

10:41 min

February 17, 2017

, , ,

Chapters

  • 00:05Title
  • 00:57Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
  • 03:42Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
  • 05:15In Vitro Heparin Binding Assay
  • 08:07Results: Representative GFP-TMEM184A-transfection Effects
  • 09:44Conclusion

Summary

Automatic Translation

与羧基末端设计用于真核表达GFP的标签的构建编码TMEM184A,在旨在证实TMEM184A的鉴定如在血管细胞肝素受体测定中使用。

Related Videos

Read Article