JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
用GFP标记TMEM184A构建肝素受体身份确认
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
Please note that all translations are automatically generated.
Click here for the English version.
用GFP标记TMEM184A构建肝素受体身份确认
DOI:
10.3791/55053-v
•
10:41 min
•
February 17, 2017
•
Sara Lynn N. Farwell
,
Joshua B. Slee
,
Yaqiu Li
,
Linda J. Lowe-Krentz
1
Department of Biological Sciences
,
Lehigh University
,
2
Department of Natural Science
,
DeSales University
Chapters
00:05
Title
00:57
Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
03:42
Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
05:15
In Vitro
Heparin Binding Assay
08:07
Results: Representative GFP-TMEM184A-transfection Effects
09:44
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
与羧基末端设计用于真核表达GFP的标签的构建编码TMEM184A,在旨在证实TMEM184A的鉴定如在血管细胞肝素受体测定中使用。
Tags
GFP-tagged
TMEM184A Construct
Heparin Receptor
Functional Identification
Orphan Genes
Transfection
Cell Culture
Rhodamine Heparin
Confocal Microscopy
Fluorescence Imaging
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
程序的微生物实验室自适应演化的恒化
单细胞测序拷贝数改变的检测
用新的转介导法测定
大肠杆菌
中 DNA 元素的最佳染色体位置 (s)
高分辨率融合PCR用于补体受体1长度多态性基因分型:阿尔茨海默病基因易感性评估的创新工具
血栓烷 A2 受体基因 C924T 多态性的研究方法
一种基于昆虫蜕皮激素受体的奇异基因开关, 用于对转基因进行有鲁棒性、可逆性和可忽略的泄漏的刻意表达
利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
CRISPR/Cas9 诱导人多潜能干细胞内源蛋白标记的应用
qkat: 杀手细胞免疫球蛋白样受体基因的定量半自动分型
基于质谱的蛋白质组学分析, 利用 OpenProt 数据库揭开从非规范开放阅读框架翻译的新蛋白质
Read Article