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Ved hjælp af en GFP-mærket TMEM184A Construct til Bekræftelse af Heparin receptor Identity
JoVE Journal
Genetics
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JoVE Journal Genetics
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
DOI:

10:41 min

February 17, 2017

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Chapters

  • 00:05Title
  • 00:57Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
  • 03:42Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
  • 05:15In Vitro Heparin Binding Assay
  • 08:07Results: Representative GFP-TMEM184A-transfection Effects
  • 09:44Conclusion

Summary

Automatic Translation

En konstruktion, der koder TMEM184A med et GFP-tag ved carboxyterminalen designet til eukaryot ekspression, blev anvendt i assays designet til at bekræfte identifikationen af ​​TMEM184A som heparin receptor i vaskulære celler.

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