Forskolin indotta Gonfiore intestinale organoidi: An<em> In Vitro</em> Test per la valutazione della risposta farmacologica nella fibrosi cistica pazienti
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.