Forskoline-geïnduceerde zwelling in Intestinal Organoids: An<em> In Vitro</em> Assay voor het beoordelen van de Drug Response in patiënten met cystische fibrose
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.